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| 产品名称： | HEL |
|---|---|
| 商品货号： | TS258780 |
| <strong>Cell line </strong>： | HEL |
| DSMZ no. ： | TS258780 |
| Species ： | confirmed as human originally with IEF of AST, MDH, NP and later by STR typing |
| Cell type ： | erythroleukemia |
| Origin ： | established from the peripheral blood of a 30-year-old man with erythroleukemia (AML M6) in relapse (after treatment for Hodgkin lymphoma) in 1980; cells were described in the literature to be capable of spontaneous and induced globin synthesis and to carry the JAK2 V617F mutation. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14736, 14871, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, large to occasionally giant polynucleated, single cells in suspension; few cells adherent; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.2-1.0 x 106 cells/ml; split 1:3 to 1:5 every 3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 36 hours |
| Harvest ： | maximal cell density at 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, CD41a +, CD71 +, CD235a +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 2.3% polyploidy - 63(60-64)<3n>XYY, -2, -9, -10, -10, -11, -14, -16, -16, -17, -19, +20, +21, +2mar, del(2)(q32), t(3;6)(p13;q16), der(5)t(5;17)(q10;q10), der(6)t(1;6)(p13;p21), der(7)add(7)(p14;q32), add(8)(p21), der(9)t(9;?)(?;11)(p24;?)(?;q13), del(11)(q13), add(15)(p11), del(20)(q13), r(20)(p11q11), dup(21)(q11q22.3-qter), psu dic(22;9)t(9;?)(?;22)(p24;?)(?;p11-13) - resembles published karyotype - carries also masked 5q- and 20q- consistent with AML M6/MDS |
| Molec. Genetics ： | expression of mutated JAK2 confirmed by RT-PCR and sequencing |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |