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| 产品名称： | JVM-2 |
|---|---|
| 商品货号： | TS258782 |
| <strong>Cell line </strong>： | JVM-2 |
| DSMZ no. ： | TS258782 |
| Species ： | confirmed as human with IEF of MDH, NP |
| Cell type ： | chronic B cell leukemia |
| Origin ： | established from the peripheral blood of a 63-year-old woman with B-prolymphocytic leukemia (B-PLL) at diagnosis; recent molecular biological characterization assigned this cell line to the entity "mantle cell lymphoma" (MCL); cell line was established by EBV-transformation during treatment with phorbol ester TPA; cells were described in the literature to have immunoglobulin light chain restriction (lambda) and to carry t(11;14) leading to IGH-CCND1 (BCL1) fusion gene and CCND1 activation. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14760, 14759, 15229, 18187 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoblastoid, round to polygonal cells growing in clusters in suspension; image; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.1-0.5 x 106 cells/ml; cells might grow slowly after thawing; in log-phase split 1:2 to 1:4 every 2-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | maximal density at about 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human pseudodiploid karyotype with 1.8% polyploidy - 46(42-46)<2n>XX, t(1;15)(q32.3;q22.1), der(8)t(3;8)(q13;p21), t(11;14)(q13;q32), dup(16)(p11p13.2) - resembles published karyotype - carries t(11;14) associated with CCND1 (cyclin D1) activation |
| Molec. Genetics ： | expression of fusion gene IGH-CCND1 (BCL1) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |