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| 产品名称： | U-266 |
|---|---|
| 商品货号： | TS258786 |
| <strong>Cell line </strong>： | U-266 |
| DSMZ no. ： | TS258786 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP and recently by STR fingerprinting |
| Cell type ： | multiple myeloma |
| Origin ： | established from the peripheral blood of a 53-year-old man with IgE-secreting myeloma (refractory, terminal) in 1968; cells were described in the literature to produce IgE lambda; possible fusion partner for hybridoma production; cells express mRNA for BCL2 gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14486, 14805, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polygonal, single or clustered cells in suspension, some loosely adherent; image; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-0.7 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days; maintain at 0.2-1.0 x 106 cells/ml; cells grow partially (loosely) adherent and can be removed with a bent Pasteur pipette (trypsin is not necessary) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 55 hours |
| Harvest ： | saturation density at 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD5 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 (+), CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, smlambda -, cylambda +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human complex hypodiploid karyotype with 6.5% polyploidy - 44(40-46)<2n>XY, -8, -10, -13, -15, +2mar, t(1;11)(p33;q13), add(3)(q27), t(4;11)(q?21;q23), add(7)(q32), add(8)(q24), add(9)(q34), add(10)(p14), add(14)(p11), add(17)(p11), add(18)(p12), der(22)t(15;22)(q21;q13) - 11q13 breakpoint recurrent in multiple myeloma |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |