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| 产品名称： | ML-2 |
|---|---|
| 商品货号： | TS258788 |
| <strong>Cell line </strong>： | ML-2 |
| DSMZ no. ： | TS258788 |
| Species ： | confirmed originally as human with IEF of AST, MDH, NP and later by STR-fingerprinting |
| Cell type ： | acute myelomonocytic leukemia |
| Origin ： | established from the peripheral blood of a 26-year-old man with acute myeloid leukemia (AML M4) at diagnosis of AML (following T-non-Hodgkin lymphoma and T-ALL) in 1978; carries t(6;11)(q27;q23) leading to KMT2A-AFDN (MLL-MLLT4; MLL-AF6) fusion gene; sister cell lines are ML-1 and ML-3 |
| Reference(s) ： | 14493, 16799, 14819, 14606 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells in suspension; image; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x106 cells/ml; after thawing, culture should first be started with 20% FBS in 24-well-plates, cells may not grow during the first 5-7 days and the viability may decrease to 10%; do not dilute, but exchange medium partially carefully; in logarithmic growth phase split 1:2 to 1:3 every 2-3 days; maintain at 0.5-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60 hours |
| Harvest ： | maximum density at 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near tetraploid karyotype - 92(84-94)<4n>XX, -Y, -Y, -7, -9, -10, -10, +11, +12, +12, +13, +13, -15, -16, -17, -17, +18, +18, -20, -20, +4mar, der(1)t(1;?)(p21;?)x2, del(6)(q23)x2, der(6)t(6;11)(q27;?q23)x2, ?der(11)t(6;11)(q27;?q23)/del(11)(q23)x2, der(11)t(11;?)(?->11p11->11q23:)x2, der(11)t(11;?)(q11-13;?), dup(13)(q32->qter)x2, der(18)t(5;?;18)(q21;?;q11)x2 - extensive ch 11 rearrangements (inc. 11q23, typical of M4 and M5 AML) obscure the cytogenetic picture - ML-2 may carry rare t(6;11) |
| Molec. Genetics ： | expression of fusion gene KMT2A-AFDN (MLL-MLLT4; MLL-AF6) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |