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| 产品名称： | THP-1 |
|---|---|
| 商品货号： | TS258789 |
| <strong>Cell line </strong>： | THP-1 |
| DSMZ no. ： | TS258789 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP, and recently by STR typing |
| Cell type ： | acute monocytic leukemia |
| Origin ： | established from the peripheral blood of a 1-year-old boy with acute monocytic leukemia (AML) at relapse in 1978; the cells can be used for induction of differentiation studies; the cells were described in the literature to produce lysozyme and to be phagocytic; carries t(9;11)(p21;q23) leading to KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14493, 15021, 15022, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells in suspension, partly in clusters; image; image ; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml (20% FBS and a 12- or 24-well plate could be of advantage); maintain at 0.2-1.0 x 106 cells/ml; split 1:2 to 1:3 every 3-4 days; note that after thawing cell viability may drop to about 40%, but cells will recover within a week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-50 hours |
| Harvest ： | saturation density at about 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD15 +, CD19 -, CD34 -, cyCD68 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-tetraploid karyotype - 94(88-96)<4n>XY/XXY, -Y, +1, +3, +6, +6, -8, -13, -19, -22, -22, +2mar, add(1)(p11), del(1)(q42.2), i(2q), del(6)(p21)x2-4, i(7p), der(9)t(9;11)(p22;q23)i(9)(p10)x2, der(11)t(9;11)(p22;q23)x2, add(12)(q24)x1-2, der(13)t(8;13)(p11;p12), add(?18)(q21) - carries t(9;11) associated with AML M5 |
| Molec. Genetics ： | expression of fusion gene KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |