微信小李
微信小章
产品简介
购买须知
| 产品名称： | JVM-3 |
|---|---|
| 商品货号： | TS258790 |
| <strong>Cell line </strong>： | JVM-3 |
| DSMZ no. ： | TS258790 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP, later by DNA-fingerprinting |
| Cell type ： | chronic B cell leukemia |
| Origin ： | established from the peripheral blood of a 73-year-old man with B-prolymphocytic leukemia (PLL) at diagnosis; cell line was established by EBV-transformation during treatment with phorbol ester TPA; cells express mRNA of the proto-oncogenes BCL2 and BCL3. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14760, 14895, 14759, 18187 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoblastoid cells growing singly or in clumps in suspension; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.1-0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | maximum density at 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near tetraploid karyotype without clear mode - 87-93<4n>XXYY, +3, -7, -8, -10, +12, +12, -14, -19, -19, -20, -20 - appears to be derivate of original hyperdiploid line which exhibited trisomy 12 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |