微信小李
微信小章
产品简介
购买须知
| 产品名称： | HDLM-2 |
|---|---|
| 商品货号： | TS258793 |
| <strong>Cell line </strong>： | HDLM-2 |
| DSMZ no. ： | TS258793 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP, later by STR profiling |
| Cell type ： | Hodgkin lymphoma |
| Origin ： | established from the pleural effusion of a 74-year-old man with Hodgkin lymphoma (nodular sclerosing, stage IV) in 1982. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14478, 14724, 14476, 14475, 14474, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round to polygonal cells in suspension; heterogeneity in cell size, 15-50 µm; many bi- and polynucleated cells; image; image ; image ; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml in a 24-well-plate; maintain between 0.5-1.5 x 106 cells/ml; split carefully, 1:2 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 70-75 hours |
| Harvest ： | maximal density of 2.0-2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 +, CD3 -, CD4 (+), CD13 -, CD14 -, CD15 +, CD19 -, CD21 -, CD25 +, CD30 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | remarkable human hypodiploid cell line with large number of rearrangements involving all chromosomes except ch 10 - 36(35-38)<2n>X, der(Y), breakpoints at: 1p21, 1q11, 2p14, 2q37, 3q21, 3p14, 3q11, 3q25.2, 3q27, 4q25, 5q11, 5q35, 6q15/16, 7p14, 8q21.1, 8q23, 8q24.2, 9p11, 11q13, 12p12, 12q24.3, 13p11, 13q14, 14q21, 14q23/24.2, 15p11, 15q13, 17p11, 17q25, 18p11, 19p13, 19q13, 21q21, 22q33, Ycen, Yq12 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |