微信小李
微信小章
产品简介
购买须知
| 产品名称： | KG-1 |
|---|---|
| 商品货号： | TS258796 |
| <strong>Cell line </strong>： | KG-1 |
| DSMZ no. ： | TS258796 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP later by DNA fingerprinting |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the bone marrow of a 59-year-old man with erythroleukemia that developped into acute myeloid leukemia (AML) at relapse in 1977; cells were described in the literature to form colonies in soft-agar in response to colony-stimulating factor and to form dendritic-like cells (DLC) in response to GM-CSF, IL-4, PMA (TPA), and ionomycin. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14677, 14678, 14680, 16670, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly round, single cells in suspension; image; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; maintain at 0.2-1.0 x 106 cells/ml; split 1:2 to 1:4 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 38 hours |
| Harvest ： | maximum density at 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 4.5% polyploidy - 45(42-47)<2n>X/XY, -4, +8, +8, -12, -17, -20, +2mar, der(5;17)(q10;q10)del(5)(q?11q?13), dup(7)(q12q33), del(7)(q22q35), i(8q)x2, der(8)t(6;8)(p11;q22), der(8)t(8;12)(p11;q13), der(11)t(1;11)(q13-21;p11-p13), der(16)t(?12;16)(?p13;q13/21) - resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene FGFR1OP2-FGFR1 (OP2-FGFR1) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |