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| 产品名称： | COLO-206F |
|---|---|
| 商品货号： | TS258798 |
| <strong>Cell line </strong>： | COLO-206F |
| DSMZ no. ： | TS258798 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | colon carcinoma |
| Origin ： | established from the ascites fluid of a 71-year-old man with colon carcinoma in 1975; The cell line was established from the same patient as the cell lines COLO-201 and COLO-205 |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly round cells; about 80% are adherent cells; about 2-8% spindle-shaped cells; some giant cells; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-3 x 106 cells/80 cm2; split confluent cultures about 1:6 once a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 2-3 days |
| Harvest ： | about 7-10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 10% polyploidy - 72(66-77)<2n>XX, -Y, +1, +3, +3, +5, -6, +7, -8, +9, -10, -13, -18, +20, -21, +3mar, i(1q), der(1)t(1;18)(q11;p11), der(3) t(3;?)(p21;?)x2, del(10)(q23.3), der(12)t(3;12)(q11;p13) - a small supernumerary centric fragment or ring present in 18% of cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |