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| 产品名称： | 7-TD-1 |
|---|---|
| 商品货号： | TS258801 |
| <strong>Cell line </strong>： | 7-TD-1 |
| DSMZ no. ： | TS258801 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | B cell hybridoma |
| Origin ： | murine myeloma cells fused with C57Bl/6 spleen cells; indicator cell line for IL-6 |
| Reference(s) ： | 15042 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells, growing single or clustered in suspension; image; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 5% h.i. FBS + 5% ConA spleen cell supernatant (or 10 ng/ml IL-6) + 2.5 x 10-5 M mercaptoethanol + 2 mM L-glutamine |
| Subculture ： | maintain at 0.1-0.3 x 106 cells/ml, optimal split ratio (daily) of 1:3, maximal split ratio of 1:5; for an IL-6 bioassay, we recommend to initially use 1.25-2.5 x 104 cells/ml and 100 µl in a 96-well-plate (it is advisable to determine the optimal cell density in various growth curves) |
| Incubation ： | at 37 °C with 7.5% CO2 |
| Doubling time ： | ca. 24-25 hours |
| Harvest ： | maximum density of 0.5-1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | bioassay for IL-6 (low cell densities are essential for reliable assays; try doubling cell dilutions beginning with cell densities of 2.5 x 104 cells/ml; duration of assay is commonly 2-3 days) |
| Cytogenetics ： | murine karyotype |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |