微信小李
微信小章
产品简介
购买须知
| 产品名称： | REH |
|---|---|
| 商品货号： | TS258802 |
| <strong>Cell line </strong>： | REH |
| DSMZ no. ： | TS258802 |
| Species ： | confirmed originally as human with IEF of AST, MDH, NP, later by STR fingerprinting |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the peripheral blood of a 15-year-old North African girl with acute lymphoblastic leukemia (ALL at first relapse) in 1973; carries t(12;21) leading to ETV6-RUNX1 (TEL-AML1) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14744, 14900, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small, round, single cells in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | cells may be difficult to culture! seed out at ca. 2 x 106 cells/ml with initially 20% FBS in a 24-well plate; cells can be maintained at high density (>2 x 106 cells/ml); minimum density at ca. 0.5 x 106 cells/ml; cells grow slowly; after thawing viability decreases during the first days, but cells recover afterwards; do not change medium during this time |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | maximum density at ca. 3-5 x 106 cells/ml or higher |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 -, CD38 +, cyCD79a+, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human pseudodiploid karyotype - 46(44-47)<2n>X, -X, +16, del(3)(p22), t(4;12;21;16)(q32;p13;q22;q24.3)-inv(12)(p13q22), t(5;12)(q31-q32;p12), der(16)t(16;21)(q24.3;q22) - sideline with inv(5)der(5)(p15q31),+18 - carries t(12;21) and del(12) producing respective ETV6-RUNX1 (TEL-AML1) fusion and deletion of residual ETC6 (TEL) |
| Molec. Genetics ： | expression of fusion gene ETV6-RUNX1 (TEL-AML1) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |