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| 产品名称： | WEHI-164S |
|---|---|
| 商品货号： | TS258813 |
| <strong>Cell line </strong>： | WEHI-164S |
| DSMZ no. ： | TS258813 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | fibrosarcoma |
| Origin ： | established from a fibrosarcoma chemically induced in a BALB/c mouse; cells were described in the literature to be sensitive to human cytotoxic monocytes, to tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) |
| Reference(s) ： | 14897, 15077 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblast-like cells growing as monolayers ; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent cultures at 1:3 to 1:6; cells are adherent, slapping of the back of the flask by hand will detach the cells; seed out at ca. 4 x 106 cells/80 cm2 in 10 ml medium |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-50 hours |
| Harvest ： | ca. 15 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, PCR assays |
| Cytogenetics ： | murine hypertriploid karyotype with 20% polyploidy - 60-62<3n> - supernumerary ring/acf but no centric fusion marker present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |