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| 产品名称： | WEHI-3B |
|---|---|
| 商品货号： | TS258814 |
| <strong>Cell line </strong>： | WEHI-3B |
| DSMZ no. ： | TS258814 |
| Species ： | confirmed originally as mouse by species PCRand recently as Mus musculus origin by COI DNA barcoding |
| Cell type ： | myelomonocytic leukemia |
| Origin ： | macrophage-like cells derived from a BALB/c mouse treated for tumor induction; cells produce IL-3 |
| Reference(s) ： | 14877, 14878, 15054, 15222, 18195 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small, round, single cells growing adherently and partly in suspension; image; image; image; image |
| Medium ： | 90-95% Iscoves MDM + 5-10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split confluent culture 1:2 to 1:5 twice to three times weeky; cells are trypsin-sensitive, detach mechanically after loosening by incubating in PBS (without Ca2+ or Mg2+) for 10-15 min; seed out at ca. 0.2-0.5 x 105 cells/cm2; alternatively, cells can be detached using TrypLE Express (Invitrogen) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20-30 hours |
| Harvest ： | maximum cell harvest of about 1 x 106 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, PCR assays |
| Cytogenetics ： | murine hypotetraploid karyotype with 19% polyploidy - 73/74(70-79)<4n> - 2-3 centric fusion markers present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |