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| 产品名称： | NAMALWA |
|---|---|
| 商品货号： | TS258815 |
| <strong>Cell line </strong>： | NAMALWA |
| DSMZ no. ： | TS258815 |
| Species ： | confirmed as human with IEF of AST, NP |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established from the tumor mass of an African child with Burkitt lymphoma in 1967; cells express MYC mRNA |
| Reference(s) ： | 18146 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV on handling the cells is unlikely. Additionally, this cell line carries sequences of the squirrel monkey retrovirus (SMRV). According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A, C |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoblastoid, single cells or small clusters in suspension; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split 1:2 to 1:4 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 20-30 hours |
| Harvest ： | maximum density at about 2.0 x 106 cells/ml or higher |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD38 +, cyCD79a +, HLA-DR +, sm/cyIgM +, sm/cyIgG -, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 4% polyploidy - 44(43-46)<2n>X, -Y, -5, -6, +7, -10, -13, -21, +3mar, ins(1;?)(p11;?), ins(3)(q11q29), der(5)t(1;5)(q25;q35), del(8)(p22), der(14)t(14;?)(p11;?), der(15)t(15;21)(p11;q11) - a supernumerary ring or fragment present in 8% of cells - NAMALWA marker chromosomes present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV + |