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| 产品名称： | MOLT-16 |
|---|---|
| 商品货号： | TS258819 |
| <strong>Cell line </strong>： | MOLT-16 |
| DSMZ no. ： | TS258819 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP, PEP B |
| Cell type ： | T cell leukemia |
| Origin ： | established from the peripheral blood of a 5-year-old girl with T cell acute lymphoblastic leukemia (T-ALL) at relapse in 1984 (sister cell line MOLT-17, ACC 36) |
| Reference(s) ： | 14477, 14740 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to elongated single cells growing in suspension; image; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split 1:2 to 1:3 twice a week; a significant percentage of the cells may die within the first 24 hours after thawing; we recommend to initiate cell culture in 12- or 24-well plates in the presence of 20% FBS |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | saturation density at 2-2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 +, CD3 +, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -; image |
| Fingerprint ： | same DNA profile as MOLT-17 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human near diploid karyotype - 46(43-47)<2n>XX, t(3;11)(p21;p13), t(8;14)(q24;q11) - t(8;14)(q24;q11) and 11p13 breakpoint are associated with T-ALL (identical to MOLT-17) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |