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| 产品名称： | VERO-B4 |
|---|---|
| 商品货号： | TS258820 |
| <strong>Cell line </strong>： | VERO-B4 |
| DSMZ no. ： | TS258820 |
| Species ： | Chlorocebus aethiops (Cercopithecus aethiops) origin confirmed by COI DNA barcoding |
| Cell type ： | kidney |
| Origin ： | established from the kidney of a normal adult African green monkey in Japan in 1962; cells are susceptible to a number of viruses; used in virus replication studies and plaque assays, and as indicator cell line for mycoplasma testing |
| Reference(s) ： | 15165 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A, E |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent-elongated, fibroblast-like cells in monolayers; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/80 cm2; split confluent culture 1:3 to 1:10 using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 25 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Fingerprint ： | unique Hinf I-(gtg)5 DNA profile |
| Cytogenetics ： | simian rearranged flat-moded hypodiploid karyotype with 12% polypoidy - 54(48-61)<2n>X - VERO-B4 markers present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, SMRV - |