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| 产品名称： | P12-ICHIKAWA |
|---|---|
| 商品货号： | TS258821 |
| <strong>Cell line </strong>： | P12-ICHIKAWA |
| DSMZ no. ： | TS258821 |
| Species ： | originally confirmed as human with IEF of AST, MDH |
| Cell type ： | T cell leukemia |
| Origin ： | established from the peripheral blood of a 7-year-old boy with acute lymphoblastic leukemia (ALL) |
| Reference(s) ： | 14664, 15055, 15217 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, polymorph cells in suspension; image; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.3-0.9 x 106 cells/ml; optimal split ratio 1:3 to 1:5; culture in 12- or 24-well plates could be of advantage |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | maximum density at about 1.2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA, PCR hybridization assays |
| Immunology ： | CD2 +, cyCD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype with 1.6% polyploidy - 82-84<4n>XX, -Y, -Y, -2, +6, -9, -9, -10, -14, +20, -21, -21, -22, -22, +mar, der(2;9)(p10;q10), del(4)(q25), der(10;22)(q10;q10), add(19)(q13) - sideline with N4, add(8)(p1?) - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |