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| 产品名称： | JOSK-M |
|---|---|
| 商品货号： | TS258822 |
| <strong>Cell line </strong>： | JOSK-M |
| DSMZ no. ： | TS258822 |
| Species ： | confirmed as human with IEF of G6PD, MDH, NP |
| Cell type ： | histiocytic lymphoma (derivative of U-937) |
| Origin ： | supposedly "established from the peripheral blood of a 37-year-old man with chronic myeloid leukemia (CML) in myelomonocytic blast crisis in 1984"; however, DSMZ DNA fingerprinting and cytogenetic analysis established unequivocally that JOSK-I, JOSK-M and U-937 are identical; thus, JOSK-M is clearly a derivative of U-937 |
| Reference(s) ： | 14491, 14815 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round to oval cells in suspension; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.2-0.3 x 106 cells/ml; subculture at a 1:4 to 1:5 dilution |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cells grow up to about 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, micobiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR + |
| Fingerprint ： | same DNA profile as U-937 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hyperdiploid with 4% polyploidy - 55(51-57)<2n>XXY, +X, +3, +7, +8, +19, +20, +3mar, t(1;5;7)(p21-22;q35;p11), der(3)t(1;3)(q21;q26-27), der(6)t(6;?)(p25;?), der(10)t(10;?)(p13;?), del(11)(q22-23), der(12)t(12;?)(p13;?), der(15)t(15;?)(q24;?) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |