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| 产品名称： | 4H1-A7 |
|---|---|
| 商品货号： | TS258826 |
| <strong>Cell line </strong>： | 4H1-A7 |
| DSMZ no. ： | TS258826 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | hybridoma (anti-canine adenovirus 2) |
| Origin ： | established by fusion of SP2/0-AG14 myeloma cells with BALB/c mouse spleen cells immunized with purified canine adenovirus type 2 (CAV-2, Manhattan strain); the secreted antibody (IgG2a) binds to and neutralizes CAV-2 and is type-specific |
| Reference(s) ： | 15059, 15060 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single cells in suspension; image; image |
| Medium ： | 92.5% Dulbeccos MEM (4.5 g/L glucose) + 5% horse serum + 2.5% h.i. FBS |
| Subculture ： | maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Harvest ： | maximum density at 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 75% medium, 10% horse serum, 5% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics ： | murine hypertetraploid/pentaploid karyotype with 5% polyploidy - 91-98<4n> - 3 centric fusion markers and 1-3 dmin present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |