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| 产品名称： | 380 |
|---|---|
| 商品货号： | TS258830 |
| <strong>Cell line </strong>： | 380 |
| DSMZ no. ： | TS258830 |
| Species ： | confirmed as human with IEF of MDH, NP |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the peripheral blood of a 15-year-old boy with acute lymphoblastic leukemia (ALL FAB L2) in relapse in 1983; cells were described in the literature to be EBNA-negative and to carry t(8;14) and t(14;18) leading to IGH-BCL2 and MYC-IGH fusion genes, respectively |
| Reference(s) ： | 14841 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, small, single cells in suspension; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | cells are very difficult to culture and grow very slowly! cells appear to grow better in 24-well-plates than in flasks; seed out at ca. 1 x 106 cells/ml; optimal density at about 1-3 x 106 cells/ml; split carefully |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80 hours |
| Harvest ： | maximal density at about 4-5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cy IgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 1.8% polyploidy - 46(43-47)<2n>XY, -14, t(8;14;18)(q24;q32;q21), +der(14)t(8;14;18)(q24;q32;q21) - corresponds to published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |