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| 产品名称： | PF-382 |
|---|---|
| 商品货号： | TS258832 |
| <strong>Cell line </strong>： | PF-382 |
| DSMZ no. ： | TS258832 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP and recently by STR-fingerprinting |
| Cell type ： | T cell leukemia |
| Origin ： | established from the pleural effusion of a 6-year-old girl with acute lymphoblastic leukemia (ALL) in second relapse |
| Reference(s) ： | 14842 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round to oval cells in suspension; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-2.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | maximal density of about 3-4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 +, CD3 -, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 +, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 9% near-tetraploid and 15% polyploid sidelines - 45/46(43-47)<2n>X/XX, -10, +14 - sideline with +add(?15)(p11), add(1)(p32) - identity confirmed |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |