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| 产品名称： | KMOE-2 |
|---|---|
| 商品货号： | TS258833 |
| <strong>Cell line </strong>： | KMOE-2 |
| DSMZ no. ： | TS258833 |
| Species ： | originally confirmed as human with IEF of AST, MDH |
| Cell type ： | acute erythremia |
| Origin ： | established from the peripheral blood of a 2-year-old girl with acute erythremia (Di Gugliemos disease, erythroblastosis) at diagnosis in 1978; cells were described in the literature to produce hemoglobin |
| Reference(s) ： | 14823 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, large, round to oval cells in suspension ; image ; image ; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.4 x 106 cells/ml; maintain at 0.2-0.3 x 106 cells/ml; note that after thawing cell viability may be as low as 50%, but cells will recover quickly within a few days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | doubling of ca. 40-50 hours |
| Harvest ： | maximal density of 0.6 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD15 -, CD19 -, CD33 +, CD34 -, CD71 +, CD235a -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotetraploid karyotype (mode weak) with 6% polyploidy - 82(80-88)<4n>XX, -X, -X, -10, -13, -14, -16, -18, -20, -22, +3mar, der(1)t(1;?)(q21;?)x2, del(7)(p15)x2, del(9)(p12), i(17q) - karyotype appears to represent derivative of that originally reported |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |