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| 产品名称： | 5637 |
|---|---|
| 商品货号： | TS258836 |
| <strong>Cell line </strong>： | 5637 |
| DSMZ no. ： | TS258836 |
| Species ： | confirmed originally as human with IEF of AST, MDH, later by STR-typing |
| Cell type ： | urinary bladder carcinoma |
| Origin ： | established from the primary bladder carcinoma of a 68-year-old man in 1974; cells were described in the literature to produce several growth factors (e.g. SCF, IL-1, IL-6, G-CSF, GM-CSF, etc.) |
| Reference(s) ： | 14539, 14643, 14867, 15057, 15203 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent cultures 1:4 to 1:5 using trypsin/EDTA (cells should be incubated for 5 to 10 min with trypsin/EDTA at 37 °C) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | about 8-10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 17% polyploidy - 62(47-64)<3n>XX, -Y, -4, -5, -10, -13, -13, -15, -18, -19, -21, -21, +4mar, der(1)t(1;?)(p21;?), der(3)t(3;?)(p22;?), del(6)(q23), der(9)t(9;?)(p12;?), der(11)t(5;11)(q14;p12), ?der(12)t(12;?)(q14;?), i(13q), i(14q), der(17)t(17;?)(p11/12;?), del(22)(q13) - carries large submetacentric marker with q arm resembling tandem dup(7) - 9p12 is a recurrent breakpoint in bladder carcinoma |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |