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| 产品名称： | 697 |
|---|---|
| 商品货号： | TS258838 |
| <strong>Cell line </strong>： | 697 |
| DSMZ no. ： | TS258838 |
| Species ： | confirmed originally as human with IEF of AST, MDH, NP, later by STR typing |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the bone marrow of a 12-year-old boy with acute lymphoblastic leukemia (cALL) at relapse in 1979; cells express BCL2, BCL3 and MYC mRNA; recently termed "EU-3" in the literature. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14533, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells and clusters of cells in suspension; image; image; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1.0 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split saturated culture at 1:3 every 2-3 days; it is of advantage to start in 24-well-plates |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30-40 hours |
| Harvest ： | maximal density at about 2-3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 -, CD38 +, CD80 -, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near diploid karyotype - 46(45-48)<2n>XY, t(1;19)(q23;p13), del(6)(q21) |
| Molec. Genetics ： | expression of fusion gene TCF3-PBX (E2A-PBX) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |