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| 产品名称： | LP-1 |
|---|---|
| 商品货号： | TS258840 |
| <strong>Cell line </strong>： | LP-1 |
| DSMZ no. ： | TS258840 |
| Species ： | confirmed originally as human with IEF of AST, MDH, NP and subsequently by STR fingerprinting |
| Cell type ： | multiple myeloma |
| Origin ： | established from the peripheral blood of a 56-year-old woman with multiple myeloma (IgG, EBV-negative) in leukemic transformation (refractory, terminal) in 1986. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14486, 14843, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, elongated, snake-like cells in suspension; image; image; image |
| Medium ： | 80-90% Iscoves MDM + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.7 x 106 cells/well; maintain at 0.5-1.0 x 106 cells/ml; split 1:2 every 2-3 days; viability may be low initially after thawing; it is recommended to cultivate the cells in 24-well-plates during the first week and to exchange medium without dilution; cells may be partially (lightly) adherent but can be flushed off by pipetting |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | 35-80 hours (depending on cell density) |
| Harvest ： | maximal density at about 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded aneuploid hypertriploid karyotype - 79(72-79)<3n>XX, -X,-1, +3, +4, +5, +6, +8, +11, +12, -13, +14, +15, +16, +18, -19, +20, +21, +22, +dminx1-4, add(1)(p11-21), del(3)(q22), der(4)add(4)(p1?)t(4;11)(11;?)(q33;q13)(q24;?)x2, der(5)t(1;5)(p11;q35), del(6)(q15)x1-2, del(8)(p21)x1-2, add(9)(q34-35)x1-2, t(12;13)(p12;q11)x2 - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |