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| 产品名称： | ELM-I-1 |
|---|---|
| 商品货号： | TS258843 |
| <strong>Cell line </strong>： | ELM-I-1 |
| DSMZ no. ： | TS258843 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | erythroleukemia |
| Origin ： | established from an X-irradiated C3H/HeNSIc mouse; cells were described in the literature to be stroma-independent, responsive to erythropoietin and to produce hemoglobin (augmented by IL-3) |
| Reference(s) ： | 14958, 14632 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large, round, single cells in suspension |
| Medium ： | 90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10% h.i. FBS |
| Subculture ： | maintain at 0.1-0.5 x 106 cells/ml; split saturated culture 1:3; maximal density at about 2-5 x 106 cells/ml; seed out at ca. 0.2-0.4 x 106 cells/ml; note that after thawing cell viability may be as low as 50%, but cells will recover quickly within a week or so |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | 20-40 h |
| Harvest ： | saturation density of about 0.5-1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |