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| 产品名称： | CTV-1 |
|---|---|
| 商品货号： | TS258847 |
| <strong>Cell line </strong>： | CTV-1 |
| DSMZ no. ： | TS258847 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | T cell leukemia |
| Origin ： | allegedly established from the peripheral blood of a 40-year-old man with acute monoblastic leukemia (AML M5) at relapse in 1982 (according to original publication allegedly derived from a woman); however, recent new data suggest derivation from T-ALL (acute lymphoblastic leukemia): (1) T-ALL-associated gene expression signature (Andersson et al., Leukemia 19: 1042-1050, 2005); (2) own FISH analysis confirming t(1;7) effecting TAL1-TRB fusion; (3) own reanalysis of the immunoprofile |
| Reference(s) ： | 14357, 14428 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round or multiformed cells, singly or clustered in suspension; image; image; image; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | maintain at 0.5-1.0 x 106 cells/ml; seed out at ca. 0.8-1.0 x 106 cells/ml; split 1:2 to 1:4 every 3-4 days; after thawing, culture should first be started with 20% FBS and best in 24-well-plates |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | maximal density at about 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, cyCD3 +, smCD3 -, CD4 -, CD5 -, CD6 (+), CD7 +, CD8 -, CD13 -, CD15 +, CD19 -, CD33 -, CD34 -, HLA-DR -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near tetraploid karyotype with 9% polyploidy - 92(89-94)<4n>XX/XXY/XXYY, -1, +6, +6, -17, t(1;7)(p34.2;q34)x2, del(3)(p21), i(6q)x2, t(12;16)(q24.32;q11)x2 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |