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| 产品名称： | DA-1 |
|---|---|
| 商品货号： | TS258849 |
| <strong>Cell line </strong>： | DA-1 |
| DSMZ no. ： | TS258849 |
| Species ： | originally identified as mouse by species PCR and later confirmed by COI DNA barcoding as mus musculus and by STR as derived from a C3H strain |
| Cell type ： | lymphoma |
| Origin ： | IL-3 dependent mast cell line established by transformation with MuLV; apparently not derived from a BALB/c mouse as described, but most likely from a C3H strain; described in the literature to be growth-dependent on IL-3, but to recognize also GM-CSF |
| Reference(s) ： | 14851 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension; image; image |
| Medium ： | 95% Iscoves MDM + 5% h.i. FBS + 2 mM L-glutamine + 2.5 x 10-5 M mercaptoethanol + IL-3 (either 30% vol conditioned medium of WEHI-3B cell line (DSM ACC 26) or 20 U/ml mIL-3) |
| Subculture ： | maintain at 0.2-0.4 x 106 cells/ml; split 1:2 to 1:3 every 1-2 days, cells grow fast. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Harvest ： | maximal density at 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, PCR assays |
| Viruses ： | ELISA: reverse transcriptase negative |