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| 产品名称： | OPM-2 |
|---|---|
| 商品货号： | TS258853 |
| <strong>Cell line </strong>： | OPM-2 |
| DSMZ no. ： | TS258853 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP and recently by STR fingerprinting |
| Cell type ： | multiple myeloma |
| Origin ： | established from the peripheral blood of a 56-year-old woman with multiple myeloma (IgG lambda) in leukemic phase (relapse, terminal) in 1982; described in the literature to carry t(4;14) leading to IGH-FGFR3 (IGH-MMSET) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14650, 14486, 18147, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round to polygonal cells in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml in a 12- or 24-well-plate; maintain at 0.3-0.7 x 106 cells/ml; split 1:2 to 1:4 every 2-3 days; viability may decrease after thawing, keep at 20% FBS for the first week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | maximal density at 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid/hypotetraploid karyotype - 78(77-82)<3n>XX-X, +1, +1, +1, +2, +4, +5, -7, +9, +11, -14, +15, +16, +18, +19, -21, +2-3mar, add(1)(p11), add(1)(q11)x2, del(1)(p11)x2, del(1)(q32), t(4;14)(p16;q32.3), der(4)t(4;14)(p16;q32.3), add(5)(q11), add(5)(q15-21), add(5)(q35), der(8)t(1;8)(?q32;q24)x1-2, add(14)(q32.1), der(22)t(1;22)(q11;p13)x2 - sideline with del(7)(p15), del(10)(q22) - resembles published karyotype - carries cryptic t(4;14) associated with fusion of IGH with FGFR3 (MMSET) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |