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| 产品名称： | KE-37 |
|---|---|
| 商品货号： | TS258857 |
| <strong>Cell line </strong>： | KE-37 |
| DSMZ no. ： | TS258857 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | T cell leukemia |
| Origin ： | established from a 27-year-old man with acute lymphoblastic leukemia (ALL) in 1979 |
| Reference(s) ： | 14473, 15220 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension; image; image; image; image |
| Medium ： | 90-92.5% RPMI 1640 + 7.5-10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/ml; after thawing, cells should be cultured with 20% FBS for the first 1-2 weeks; then maintain at about 1-1.5 x 106 cells/ml and split saturated culture 1:2 to 1:3 every second day; maximum density at ca. 3 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 2-3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 +, cyCD3 +, smCD3 -, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype - 91(86-92)<4n>XXYY, +8, -14, -14, t(7;12)(q32-33;p12-13)x2, der(8)t(8;14)(q24;q11)x4, der(14)t(8;14)(q24;q11)x2 - resembles published karyotype - carries 4 copies of der(8)t(8;14) associated with T-ALL - normal ch 14 present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |