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| 产品名称： | CAKI-2 |
|---|---|
| 商品货号： | TS258859 |
| <strong>Cell line </strong>： | CAKI-2 |
| DSMZ no. ： | TS258859 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | kidney carcinoma |
| Origin ： | established from the primary renal carcinoma of a 69-year-old man |
| Reference(s) ： | 14537, 14538 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells, growing as adherent monolayer |
| Medium ： | 90% McCoys 5A + 10% h.i. FBS |
| Subculture ： | change medium twice every week, split confluent cultures 1:2 to 1:3 weekly using trypsin/EDTA, upon thawing grow cells immediately in 80 cm2; seed out at ca. 2 x 106 cells/80 cm2 in 10 ml medium |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 1 x 106 cells/25 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded pentaploid/hexaploid karyotype with 12% polyploidy - 130(118-132)<6n>XXX, -Y, -Y, -Y, inc, add(1)(p22), i(8q), i(?5q)x2, del(6)(q23), t(14;15)(p10;q10), der(19)t(1;19)(q24;q13), dminx1, large iso der(5q)x2, and submeta marker der(?)t(?;2)(?;q11)x2 - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |