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| 产品名称： | SKW-3 |
|---|---|
| 商品货号： | TS258862 |
| <strong>Cell line </strong>： | SKW-3 |
| DSMZ no. ： | TS258862 |
| Species ： | confirmed as human with IEF of MDH, NP |
| Cell type ： | T cell leukemia (derivative of KE-37) |
| Origin ： | originally described in the literature to be established from the peripheral blood of a 61-year-old man with T cell chronic lymphocytic leukemia (CLL) in 1977; however, DNA fingerprinting and cytogenetic analysis showed cross-contamination with cell line KE-37; KE-37 was established from a 27-year-old man with acute lymphoblastic leukemia (ALL) in 1979 |
| Reference(s) ： | 14610, 14473 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | saturation density at about 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, PCR assays |
| Immunology ： | CD2 +, cyCD3 +, CD3 -, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | same DNA profile as KE-37 by fluorescent nonaplex PCR of short tandem repeat markers |
| Cytogenetics ： | human near diploid karyotype with 4% polyploidy - 46(43-48)<2n>XY, +8, -14, t(3;3)(q11;q27), der(8)t(8;14)(q24;q11)x2, t(8;11)(p21;p12), der(12)t(12;?)(q24;?), der(14)t(8;14)(q24;q11) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |