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| 产品名称： | R1 |
|---|---|
| 商品货号： | TS258863 |
| <strong>Cell line </strong>： | R1 |
| DSMZ no. ： | TS258863 |
| Species ： | Oncorhynchus tshawytscha origin determined by COI DNA barcoding |
| Cell type ： | liver |
| Origin ： | established from the liver of a rainbow trout; however, COI DNA barcoding revealed R1 as originating from a Chinook salmon (Oncorhynchus tshawytscha) and not from rainbow trout (Oncorhynchus mykiss) as described |
| Reference(s) ： | 14350 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, fibroblastoid cells growing as monolayer |
| Medium ： | 90% Medium 199 (25 mM HEPES) with Earles salts with L-glutamine + 10% h.i. FBS |
| Subculture ： | cell growth is extremely slow; split confluent culture 1:2 every 7 days using TNE (50 mM Tris, 0.1 M NaCl, 5 mM EDTA, pH 7.5) at +4 °C for 10 min; culture at room temperature (about 20 °C) without CO2 (keep lid of flask closed); cells can be stored temporarily (up to 1 week) at 4 °C; seed out at ca. 0.1 x 106 cells/ml |
| Incubation ： | at room temperature (about 20 °C) without CO2 |
| Doubling time ： | about 160 hours |
| Harvest ： | about 5 x 106 cells/175 cm2 - saturation density of ca. 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated by the depositor with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | PCR: SMRV - |