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| 产品名称： | WSU-NHL |
|---|---|
| 商品货号： | TS258869 |
| <strong>Cell line </strong>： | WSU-NHL |
| DSMZ no. ： | TS258869 |
| Species ： | confirmed as human with IEF of MDH, NP, PEP B |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the pleural effusion of a 48-year-old woman with nodular histiocytic lymphoma (refractory, progressive) in 1986; reported to carry a t(14;18) leading to the IGH-BCL2 fusion gene and to express mRNA for the BCL2 oncogene; assigned to GCB-like lymphoma subtype (germinal center B-cell) |
| Reference(s) ： | 14773, 17354 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to elongated cells in suspension, single or in small clusters; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-0.8 x 106 cells/ml; maintain at 0.5-2.0 x 106 cells; split 1:2 to 1:4 every 2-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 45 hours |
| Harvest ： | maximum density at about 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +, sm/cykappa -, sm/cylambda +, sm/cyIgG +, sm/cyIgM -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 6% polyploidy - 45(42-45)<2n>XX, -4, -15, +mar, der(1)t(1;4)(p36;q11), der(1)t(1;?7)(p34;q31.2)t(1;16)(q22;q22), del(5)(q32q35), der(6)t(1;6)(q22;p21), t(9;13)(p22;q13-14), der(13)t(13;?16)(p11;p11), t(14;18)(q32;q21), der(16)t(16;?)(q22;?)t(?;5)(?;q31), add(17)(p1?), add(19)(q13.2) - matches published karyotype - carries t(14;18) associated with NHL |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |