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| 产品名称： | COS-7 |
|---|---|
| 商品货号： | TS258876 |
| <strong>Cell line </strong>： | COS-7 |
| DSMZ no. ： | TS258876 |
| Species ： | Chlorocebus aethiops (Cercopithecus aethiops) origin confirmed by COI DNA barcoding |
| Cell type ： | kidney |
| Origin ： | derived from CV-1, a simian cell line (cercopithecus aethiops), by transformation with an origin-defective mutant of SV-40; cells were described in the literature to support the growth of SV-40 viruses; classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS) |
| Reference(s) ： | 14568 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | The cell line was established by transfection with a mutated simian virus 40 (SV40) exhibiting a 6 bp deletion in the replication origin. This renders the virus incompetent for replication and the cell line can thus be handled under biosafety level 1. |
| Permissions and restrictions ： | A, D, E |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblast-like cells growing as monolayers ; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-48 hours |
| Harvest ： | about 2-3 x 106 cells/80 cm2; saturation density at about 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Fingerprint ： | unique Hinf I-(gtg)5 DNA profile (identical to COS-1, DSM ACC 63) |
| Cytogenetics ： | simian hypodiploid karyotype with 25% polyploidy - 57(53-58)<2n> |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, SMRV - |