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| 产品名称： | MDA-MB-453 |
|---|---|
| 商品货号： | TS258879 |
| <strong>Cell line </strong>： | MDA-MB-453 |
| DSMZ no. ： | TS258879 |
| Species ： | originally confirmed as human with IEF of MDH, NP and recently by STR fingerprinting |
| Cell type ： | breast carcinoma |
| Origin ： | established from an effusion of a 48-year-old woman with breast carcinoma in 1976 |
| Reference(s) ： | 14415, 14960 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like, round, loosely attached monolayers; image; image |
| Medium ： | 90% Leibovitzs L-15 medium + 10% h.i. FBS |
| Subculture ： | seed out at 2-4 x 106 cells/80 cm2; split confluent culture 1:2 to 1:6 using trypsin/EDTA; cells can partially be detached by tapping the flask; cells may need several days to re-adhere again |
| Incubation ： | at 37 °C, no exposure to CO2 (keep lid of flask closed) |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | about 25-35 x 106 cells/80 cm2 |
| Storage ： | frozen with 85% medium, 10% FBS, 5% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotetraploid karyotype with 6% polyploidy and near diploid sideline (2%) - 89(85-91)<4n>XXX, -X - highly rearranged leading to extensive marker formation, including der(5)t(5;?14)(p11;q11)x2 - dm present in 12% cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |