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| 产品名称： | BT-474 |
|---|---|
| 商品货号： | TS258882 |
| <strong>Cell line </strong>： | BT-474 |
| DSMZ no. ： | TS258882 |
| Species ： | originally confirmed as human with IEF of AST, GPDH, LDH, NP and recently by STR fingerprinting |
| Cell type ： | breast ductal carcinoma |
| Origin ： | established from a solid, invasive ductal carcinoma of the breast obtained from a 60-year-old woman; cells were reportedly tumorigenic in athymic mice and were found to be susceptible to mouse mammary tumor virus; described to carry a mutation in the TP53 gene, rendering cells temperature sensitive |
| Reference(s) ： | 14706 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent patches of epithelial cells; individual colonies with a thick center and a thin smooth margin; image; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 10 µg/ml human insulin + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1-3 x 106 cells/25 cm2; split confluent culture 1:2 to 1:3 every 7-10 days using trypsin/EDTA; cells grow slowly and may produce cell debris |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 100 hours |
| Harvest ： | about 1.0-1.6 x 105 cells/cm2 or 20 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertetraploid karyotype - 104(91-111)<4n> |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |