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| 产品名称： | MEL-HO |
|---|---|
| 商品货号： | TS258885 |
| <strong>Cell line </strong>： | MEL-HO |
| DSMZ no. ： | TS258885 |
| Species ： | confirmed originally as human with IEF of AST, MDH, PEP B and later by STR fingerprinting |
| Cell type ： | melanoma |
| Origin ： | established from the primary tumor of a woman with melanoma in 1976 |
| Reference(s) ： | 14615, 15210 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells with long, narrow, often branching cell bodies; image; image; image; image; image |
| Medium ： | 95% RPMI 1640 + 5% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 or 175 cm2; split confluent culture 1:5 to 1:10 using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | about 10-12 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 1.5% polyploidy - 74(72-76)<3n>XX, -X, +3, +7, +7, -8, -10, -11, +15, -16, +17, +18, +20, +21, +mar - del(1)(q42), del(3)(p21), i(7q)x2, del(9)(p13), der(15)t(15;?)(q21;?), i(17q), del(X)(q11) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |