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| 产品名称： | BHK-21 |
|---|---|
| 商品货号： | TS258886 |
| <strong>Cell line </strong>： | BHK-21 |
| DSMZ no. ： | TS258886 |
| Species ： | originally confirmed as Syrian (Golden) Hamster by species PCR and recently as Mesocricetus auratus origin confirmed by COI DNA barcoding |
| Cell type ： | kidney |
| Origin ： | BHK-21 is a subclone (clone 13) of the parental line established from the kidneys of five unsexed, one-day-old hamsters in 1961; cells can be used for virus replication studies |
| Reference(s) ： | 14727, 14726 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblastoid adherent monolayer; image; image; image |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS + 1x non-essential amino acids |
| Subculture ： | seed out at ca. 0.2 x 105 cells/cm2; split confluent culture 1:2 to 1:3 every 2-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-50 hours |
| Harvest ： | about 10-15 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Fingerprint ： | unique DNA profile with (gtg)5 multilocus probe |
| Cytogenetics ： | near-diploid golden (Syrian) hamster karyotype with 21% tetraploidy - 40-45, XY - hyperdiploid sideline present at 19% - resembles published description |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |