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| 产品名称： | CA-46 |
|---|---|
| 商品货号： | TS258898 |
| <strong>Cell line </strong>： | CA-46 |
| DSMZ no. ： | TS258898 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established from the ascites fluid of a patient with American-type Burkitt lymphoma; cells were described in the literature to be EBNA-negative, to carry the t(8;14) and to express BCL2 and MYC mRNA |
| Reference(s) ： | 14730, 14731 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly round, some oval or pear-like cells growing singly in suspension; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at 0.5 x 106 cells/ml; maintain at 0.5-2.0 x 106 cells/ml, split 1:2 to 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 35 hours |
| Harvest ： | saturation density more than 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near diploid karyotype with about 3% triploidy - 46(45-48)<2n>X/XY, dup(1)(q21q32), dup(7)(q12q22), t(8;14)(q24;q32) - additional rearrangements were present in a sideline, viz. t(6;13)(p21;q32) and del(11)(p11) - a supernumerary dmin was present in about 25% metaphases |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |