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| 产品名称： | BA-D5 |
|---|---|
| 商品货号： | TS258899 |
| <strong>Cell line </strong>： | BA-D5 |
| DSMZ no. ： | TS258899 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | hybridoma (anti-rat fiber/myosin) |
| Origin ： | this hybridoma cell line was described in the literature to secrete a mouse monoclonal antibody (IgG) that reacts with rat type 1 fibers in immunofluorescence and with rat type 1 MHCs (myosin heavy chain) in western blotting; the cell line was established in 1986 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse that had been immunized with purified myosin from bovine atrium |
| Reference(s) ： | 14929 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension (some cells adhere partly, but trypsin is not necessary to detach these cells) |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS (or horse serum) + 2 mM L-glutamine |
| Subculture ： | split saturated culture at 1:5 every 2-3 days; optimal density at about 0.5 x 106 cells/ml; after thawing, culture first with 20% FBS, viability may drop to as low as 50-60% |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 25 hours |
| Harvest ： | cell harvest of about 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG2b monoclonal antibody |
| Cytogenetics ： | murine hypertetraploid/pentaploid karyotype with 10% polyploidy - 90(88-100)<4n> - 1-3 centric fusion markers and 2 dmin present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |