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| 产品名称： | NAMALWA.IPN/45 |
|---|---|
| 商品货号： | TS258905 |
| <strong>Cell line </strong>： | NAMALWA.IPN/45 |
| DSMZ no. ： | TS258905 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established from the tumor mass of an African child with Burkitt lymphoma; this variant subline from the original NAMALWA (ACC 24) was described in the literature to be characterized by differential expression of immunoglobulin genes, MYC genes and cell surface antigens (see reference); cells were reported to harbor a retrovirus of simian origin, SMRV-H |
| Reference(s) ： | 14584 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely. Additionally, this cell line carries sequences of the squirrel monkey retrovirus (SMRV). According to a recommendation of the German Commission for Biosafety the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A, C |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoblastoid, single cells with irregular forms or small clusters, in suspension |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | maintain at 0.5-1.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Harvest ： | maximal density of about 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | CD3 -, CD13 -, CD19 +, CD37 +, HLA-DR +, smIgM +, smIgG - |
| Fingerprint ： | same DNA profile as NAMALWA using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypodiploid karyotype - 45(42-49)<2n>X, -Y, -3, +7, ins(1;?)(q11;?), ins(3) (q11;q27-29), der(5)t(5;?)(p15;?), der(12)t(12;?)(q24;?), der(15)t(15;?)(p11;?) - NAMALWA marker chromosomes present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV + |