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| 产品名称： | L-540 |
|---|---|
| 商品货号： | TS258906 |
| <strong>Cell line </strong>： | L-540 |
| DSMZ no. ： | TS258906 |
| Species ： | confirmed originally as human with IEF of AST, LDH |
| Cell type ： | Hodgkin lymphoma |
| Origin ： | established from the bone marrow of a 20-year-old woman with Hodgkin lymphoma (nodular sclerosis; stage IVB, pre-terminal stage) |
| Reference(s) ： | 14462, 14463, 14478, 15213 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph cells growing singly in suspension, heterogeneity in cell size; image; image; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; after thawing culture first in 24-well-plates; cells do not grow during the first 2-3 days after thawing and viability may drop to 50-60%; in logarithmic growth split saturated culture 1:2 every 3 days, exchange spent medium every 1-2 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | saturation density at about 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 +, CD3 -, CD13 -, CD14 -, CD15 +, CD19 -, CD21 -, CD25 +, CD30 +, CD34 -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 16% polyploidy - 59-64<3n>XX, -X, -1, +2, -6, +7, -8, -9, -10, -11, -13, -14, -16, -17, -18, +22, +5-6mar, der(1;5)(q10;p10)x1-2, der(2;12)(q10;q10), der(2)t(2;?8)(q3?7;q2?1)x1-2, ins(5;?)(p11;?), add(7)(p1?), add(8)(q24)x1-2, add(9)(p1?), add(11)(q24), der(15)t(1;15)(p21;p13)x2, add(19)(p11), add(21)(p13) |
| Molec. Genetics ： | expression of fusion gene TNK1-TMEM256 (TNK1-C17orf61) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |