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| 产品名称： | EB-1 |
|---|---|
| 商品货号： | TS258909 |
| <strong>Cell line </strong>： | EB-1 |
| DSMZ no. ： | TS258909 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established from the maxilla tumor lymph node of a 9-year-old black girl with Burkitt lymphoma in 1964; cells were described in the literature to be EBV-positive |
| Reference(s) ： | 14518, 14519, 14520 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single or aggregated cells in suspension; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml; split 1:2 to 1:3 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30-40 hours |
| Harvest ： | maximal density at 1.0-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 15% polyploidy - 47(44-48)<2n>XX/XXX, +7, t(8;14)(q24;q32), der(11)t(11;?)(q22;?), der(19)t(19;?)(q13;?) - sideline carries additional ch 2 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |