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| 产品名称： | DAUDI |
|---|---|
| 商品货号： | TS258911 |
| <strong>Cell line </strong>： | DAUDI |
| DSMZ no. ： | TS258911 |
| Species ： | originally confirmed as human with IEF of AST, NP, PEP B, later by STR typing |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established from the Burkitt lymphoma (left orbital biopsy) of a 16-year-old African boy in 1967; cells were described in the literature to be EBV-positive and to express mRNA for proto-oncogene BCL2. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14668, 14794, 18187 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells in suspension; image 1; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/ml; cells may grow slowly initially; cell culture may be started in 24-well-plates and later transfered to flasks; maintain at 0.5-1.0 x 106 cells/ml; split ratio 1:2 to 1:4 every 2-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 48 hours |
| Harvest ： | maximal density at about 1.7 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD19 +, CD20 +, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgM +, sm/cyIgG -, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near diploid karyotype with 20% polyploidy - 46(45-48)<2n>XY/XXY, +7, -9, t(8;14)(q24;q32) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |