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| 产品名称： | D-11 |
|---|---|
| 商品货号： | TS258917 |
| <strong>Cell line </strong>： | D-11 |
| DSMZ no. ： | TS258917 |
| Species ： | Oncorhynchus tshawytscha origin determined by COI DNA barcoding |
| Cell type ： | liver |
| Origin ： | this cell line is a subclone of the original cell line R1 established after elimination of mycoplasma with BM-Cyclin (tiamulin & minocycline); however, COI DNA barcoding revealed D11 (as well as R1) as originating from a Chinook salmon (Oncorhynchus tshawytscha) and not from rainbow trout as described for the R1 cell line |
| Reference(s) ： | 14350 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, fibroblastoid cells growing as monolayers |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:2 every 7-10 days using trypsin/EDTA in PBS without Ca2+/Mg2+ at room temperature for 5-10 min |
| Incubation ： | at room temperature (about 20 °C) without CO2 (keep lid of flask closed), cells can be stored temporarily (up to 1 week) at 4 °C |
| Doubling time ： | about 80 hours or more |
| Harvest ： | about 5-15 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization and PCR assays |
| Viruses ： | PCR: SMRV - |