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| 产品名称： | MOLT-3 |
|---|---|
| 商品货号： | TS258919 |
| <strong>Cell line </strong>： | MOLT-3 |
| DSMZ no. ： | TS258919 |
| Species ： | confirmed originally as human with IEF of AST, MDH, NP later by STR fingerprinting |
| Cell type ： | T cell leukemia |
| Origin ： | established from the peripheral blood of a 19-year-old man with acute lymphoblastic leukemia (ALL) in relapse in 1971; sister cell line of MOLT-4 (DSMZ ACC 362) |
| Reference(s) ： | 14767 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells in suspension ; image ; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-0.7 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 every second day |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | maximal density at about 0.7-1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 +, cyCD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | same DNA profile as MOLT-4 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypertetraploid karyotype - 98(94-101)<4n>XXYY, +6, +7, +8, +8, +17, +20, del(6)(q16)x2, der(7)t(7;7)(p15;q11)x2 - identity confirmed |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |