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| 产品名称： | MC-116 |
|---|---|
| 商品货号： | TS258920 |
| <strong>Cell line </strong>： | MC-116 |
| DSMZ no. ： | TS258920 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP, at a later time-point confirmed by STR fingerprinting |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the pleural effusion of a patient with B cell lymphoma (undifferentiated lymphoma); cells were described in the literature to be EBV-negative, to carry the t(8;14) and to express mRNA of the proto-oncogene BCL2 |
| Reference(s) ： | 14731, 14730 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to elongated, lymphoblastoid, single or clustered cells in suspension; image; image; image |
| Medium ： | 80-85% RPMI 1640 + 15-20% h.i. FBS |
| Subculture ： | maintain at 1-2 x 106 cells/ml; split ratio of 1:2 to 1:3; seed out at ca. 1 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 24-48 hours |
| Harvest ： | maximal density at 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD138 -, HLA-DR +, sm/cyIgM +, sm/cyIgG -, sm/cykappa -, sm/cylambda + |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near diploid karyotype with 1.5% polyploidy - 46(37-46)<2n>XY, dup(1)(q21q32), t(8;14)(q24;q32), del(10)(q23) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |