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| 产品名称： | DG-75 |
|---|---|
| 商品货号： | TS258922 |
| <strong>Cell line </strong>： | DG-75 |
| DSMZ no. ： | TS258922 |
| Species ： | confirmed as human with IEF of AST, MDH, PEP B |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established from the pleural effusion of a 10-year-old boy with Burkitt lymphoma (refractory, terminal) in 1975 |
| Reference(s) ： | 14377 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polygonal, single or clustered cells in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.1-0.5 x 106 cells/ml; split 1:5 to 1:10 every 24-48 hours |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | saturation density at 0.75-1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, cyIgG -, cyIgM +, cykappa +, cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near diploid karyotype with 6% polyploidy - 46<2n>XY, t(8;14)(q24;q32) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |