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| 产品名称： | COLO-818 |
|---|---|
| 商品货号： | TS258925 |
| <strong>Cell line </strong>： | COLO-818 |
| DSMZ no. ： | TS258925 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | melanoma |
| Origin ： | established from malignant melanoma |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent growth in monolayers, mostly spindle-like, some cells with cellular processes, 1-5% very big cells, no strict contact inhibition; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture about 1:6 once a week using trypsin/EDTA; seed out at about 1 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 60 hours |
| Harvest ： | cell harvest of about 8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | unique DNA profile using multiplex PCR of minisatellite markers, but identical with COLO-800 (DSM ACC 193) |
| Cytogenetics ： | human hypotriploid karyotype with 30% polyploidy - 62(57-63)<3n>X, -X, -X, -4, -5, +7, +8, -9, -10, -11, -13, +15, -16, -17, -19, -21, -21, +3mar, 1 dm present in 15% cells - add(3)(p11), der(13;21)(p10;10) - high level of subclonal rearrangement present - closely related to COLO-800 (DSM ACC 193) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |